2025
Hernández-Arciga U, Stamenkovic C, Yadav S, Nicoletti C, Albalawy WN, Al Hammood F, Gonzalez TF, Naikwadi MU, Graham A, Smarz C, Little GJ, Williams SG, McMahon B, Sipula IJ, Vandevender AM, Chuan B, Cooke D, Pinto AFM, Flores LC, Hartman HL, Diedrich JK, Brooke RT, Alder JK, Frahm KA, Pascal LE, Stolt E, Troensegaard H, Øvrebø B, Elshorbagy A, Molina E, Vinknes KJ, Tan RJ, Weisz OA, Bueno M, Eickelberg O, Steinhauser ML, Finkel T, Ables GP, Ikeno Y, Olsen T, Sacco A, Jurczak MJ, Sukoff Rizzo SJ, Parkhitko AA. Dietary methionine restriction started late in life promotes healthy aging in a sex-specific manner. Sci Adv. 2025 Apr 18;11(16):eads1532. doi: 10.1126/sciadv.ads1532. Epub 2025 Apr 16. PMID: 40238871
Gurkar AU, Okawa S, Guillermier C, Chaddha K, Steinhauser ML. Large-Scale Clustered Transcriptional Silencing Associated With Cellular Senescence. Aging Cell. 2025 Apr;24(4):e70015. doi: 10.1111/acel.70015. Epub 2025 Feb 19. PMID: 39968744
Kumar GVN, Wang RS, Sharma AX, David NL, Amorim T, Sinden DS, Doshi NK, Wabitsch M, Gingras S, Ejaz A, Rubin JP, Maron BA, Fazeli PK, Steinhauser ML. Non-canonical lysosomal lipolysis drives mobilization of adipose tissue energy stores with fasting. Nat Commun. 2025 Feb 4;16(1):1330. doi: 10.1038/s41467-025-56613-3. PMID: 39900947
2024
Zhang Y, David NL, Pesaresi T, Andrews RE, Kumar GVN, Chen H, Qiao W, Yang J, Patel K, Amorim T, Sharma AX, Liu S, Steinhauser ML. Noncoding variation near UBE2E2 orchestrates cardiometabolic pathophenotypes through polygenic effectors. JCI Insight. 2024 Dec 10:e184140. doi: 10.1172/jci.insight.184140. Epub ahead of print. PMID: 39656538.
Bomba-Warczak EK, Velez KM, Zhou LT, Guillermier C, Edassery S, Steinhauser ML, Savas JN, Duncan FE. Exceptional longevity of mammalian ovarian and oocyte macromolecules throughout the reproductive lifespan. Elife. 2024 Oct 31;13:RP93172. doi: 10.7554/eLife.93172. PMID: 39480006
Quaytman JA, David NL, Venugopal S, Amorim T, Beatrice B, Toledo FGS, Miller RG, Steinhauser ML, Fazeli PK. Intermittent fasting for systemic triglyceride metabolic reprogramming (IFAST): Design and methods of a prospective, randomized, controlled trial. Contemp Clin Trials. 2024 Nov;146:107698. doi: 10.1016/j.cct.2024.107698. Epub 2024 Sep 17. PMID: 39299543
Steinhauser ML. Unhealthy visceral fat is associated with improved efficacy of immunotherapy in endometrial cancer. J Clin Invest. 2024 Sep 3;134(17):e183675. doi: 10.1172/JCI183675. PMID: 39225094; PMCID: PMC11364398.
Guillermier C, Kumar NV, Bracken RC, Alvarez D, O'Keefe J, Gurkar A, Brown JD, Steinhauser ML. Nanoscale imaging of DNA-RNA identifies transcriptional plasticity at heterochromatin. Life Sci Alliance. 2024 Sep 17;7(12):e202402849. doi: 10.26508/lsa.202402849. PMID: 39288993; PMCID: PMC11408601.
Zhu B, Liu S, David NL, Dion W, Doshi NK, Siegel LB, Amorim T, Andrews RE, Kumar GVN, Li H, Irfan S, Pesaresi T, Sharma AX, Sun M, Fazeli PK, Steinhauser ML. Evidence for ~12-h ultradian gene programs in humans. NPJ Biol Timing Sleep. 2024;1(1):4. doi: 10.1038/s44323-024-00005-1. Epub 2024 Aug 9. PMID: 39148626; PMCID: PMC11325440.
Steinhauser ML, Maron BA. Viewing Pulmonary Arterial Hypertension Pathogenesis and Opportunities for Disease-Modifying Therapy Through the Lens of Biomass. JACC Basic Transl Sci. 2024 Jul 3;9(10):1252-1263. doi: 10.1016/j.jacbts.2024.04.009. eCollection 2024 Oct. PMID: 39534642
Rachedi NS, Tang Y, Tai YY, Zhao J, Chauvet C, Grynblat J, Akoumia KKF, Estephan L, Torrino S, Sbai C, Ait-Mouffok A, Latoche JD, Al Aaraj Y, Brau F, Abélanet S, Clavel S, Zhang Y, Guillermier C, Kumar NVG, Tavakoli S, Mercier O, Risbano MG, Yao ZK, Yang G, Ouerfelli O, Lewis JS, Montani D, Humbert M, Steinhauser ML, Anderson CJ, Oldham WM, Perros F, Bertero T, Chan SY. Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness. Cell Metab. 2024 Jun 4;36(6):1335-1350.e8. doi: 10.1016/j.cmet.2024.04.010. Epub 2024 May 2. PMID: 38701775
Bracken RC, Davison LM, Buehler DP, Fulton ME, Carson EE, Sheng Q, Stolze LK, Guillermier C, Steinhauser ML, Brown JD. Transcriptional synergy in human aortic endothelial cells is vulnerable to combination p300/CBP and BET bromodomain inhibition. iScience. 2024 May 16;27(6):110011. doi: 10.1016/j.isci.2024.110011. eCollection 2024 Jun 21. PMID: 38868181
Amorim T, Kumar NG, David NL, Dion W, Pagadala T, Doshi NK, Zhu B, Parkhitko A, Steinhauser ML, Fazeli PK. Methionine as a regulator of bone remodeling with fasting. JCI Insight. 2024 May 21;9(12):e177997. doi: 10.1172/jci.insight.177997. PMID: 38780544
2023
Massengale M, Massengale JL, Benson CR, Baryawno N, Oki T, Steinhauser ML, Wang A, Balani D, Oh LS, Randolph MA, Gill TJ 3rd, Kronenberg HM, Scadden DT. Adult Prg4+ progenitors repair long-term articular cartilage wounds in vivo. JCI Insight. 2023 Sep 8;8(17):e167858. doi: 10.1172/jci.insight.167858. PMID: 37681409
Wertheim BM, Wang RS, Guillermier C, Hütter CV, Oldham WM, Menche J, Steinhauser ML, Maron BA. Proline and glucose metabolic reprogramming supports vascular endothelial and medial biomass in pulmonary arterial hypertension. JCI Insight. 2023 Feb 22;8(4):e163932. doi: 10.1172/jci.insight.163932. PMID: 36626231
2022
Lerchenmüller C, Vujic A, Mittag S, Wang A, Rabolli CP, Heß C, Betge F, Rangrez AY, Chaklader M, Guillermier C, Gyngard F, Roh JD, Li H, Steinhauser ML, Frey N, Rothermel B, Dieterich C, Rosenzweig A, Lee RT. Restoration of Cardiomyogenesis in Aged Mouse Hearts by Voluntary Exercise. Circulation. 2022 Aug 2;146(5):412-426. doi: 10.1161/CIRCULATIONAHA.121.057276. Epub 2022 Jul 6. PMID: 35862076
Monji A, Zhang Y, Kumar GVN, Guillermier C, Kim S, Olenchock B, Steinhauser ML. A Cycle of Inflammatory Adipocyte Death and Regeneration in Murine Adipose Tissue. Diabetes. 2022 Mar 1;71(3):412-423. doi: 10.2337/db20-1306. PMID: 35040481
2021
Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O'Toole ET, Morgan GP, Ali L, Saito T, Guillermier C, Saido TC, Steinhauser ML, Stowell MHB, Chapman ER, Contractor A, Savas JN. Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals. Cell Syst. 2021 Feb 17;12(2):141-158.e9. doi: 10.1016/j.cels.2020.11.007. Epub 2020 Dec 15. PMID: 33326751
El Khoudary SR, Fabio A, Yester JW, Steinhauser ML, Christopher AB, Gyngard F, Adams PS, Morell VO, Viegas M, Da Silva JP, Da Silva LF, Castro-Medina M, McCormick A, Reyes-Múgica M, Barlas M, Liu H, Thomas D, Ammanamanchi N, Sada R, Cuda M, Hartigan E, Groscost DK, Kühn B. Design and rationale of a clinical trial to increase cardiomyocyte division in infants with tetralogy of Fallot. Int J Cardiol. 2021 Sep 15;339:36-42. doi: 10.1016/j.ijcard.2021.07.020. Epub 2021 Jul 12. PMID: 34265312
Yester JW, Liu H, Gyngard F, Ammanamanchi N, Little KC, Thomas D, Sullivan MLG, Lal S, Steinhauser ML, Kühn B. Use of stable isotope-tagged thymidine and multi-isotope imaging mass spectrometry (MIMS) for quantification of human cardiomyocyte division. Nat Protoc. 2021 Apr;16(4):1995-2022. doi: 10.1038/s41596-020-00477-y. Epub 2021 Feb 24. PMID: 33627842
Gyngard F, Trakimas L, Steinhauser ML. High-Fidelity Quantification of Cell Cycle Activity with Multi-Isotope Imaging Mass Spectrometry. Methods Mol Biol. 2021;2158:257-268. doi: 10.1007/978-1-0716-0668-1_19. PMID: 32857379
2020
Fazeli PK, Zhang Y, O'Keefe J, Pesaresi T, Lun M, Lawney B, Steinhauser ML. Prolonged fasting drives a program of metabolic inflammation in human adipose tissue. Mol Metab. 2020 Dec;42:101082. doi: 10.1016/j.molmet.2020.101082. Epub 2020 Sep 28. PMID: 32992039
Narendra DP, Steinhauser ML. Metabolic Analysis at the Nanoscale with Multi-Isotope Imaging Mass Spectrometry (MIMS). Curr Protoc Cell Biol. 2020 Sep;88(1):e111. doi: 10.1002/cpcb.111. PMID: 32706155
Zhang Y, Guillermier C, De Raedt T, Cox AG, Maertens O, Yimlamai D, Lun M, Whitney A, Maas RL, Goessling W, Cichowski K, Steinhauser ML. Imaging Mass Spectrometry Reveals Tumor Metabolic Heterogeneity. iScience. 2020 Aug 21;23(8):101355. doi: 10.1016/j.isci.2020.101355. Epub 2020 Jul 10. PMID: 32712466
Weeks O, Bossé GD, Oderberg IM, Akle S, Houvras Y, Wrighton PJ, LaBella K, Iversen I, Tavakoli S, Adatto I, Schwartz A, Kloosterman D, Tsomides A, Charness ME, Peterson RT, Steinhauser ML, Fazeli PK, Goessling W. Fetal alcohol spectrum disorder predisposes to metabolic abnormalities in adulthood. J Clin Invest. 2020 May 1;130(5):2252-2269. doi: 10.1172/JCI132139. PMID: 32202514
Narendra DP, Guillermier C, Gyngard F, Huang X, Ward ME, Steinhauser ML. Coupling APEX labeling to imaging mass spectrometry of single organelles reveals heterogeneity in lysosomal protein turnover. J Cell Biol. 2020 Jan 6;219(1):e201901097. doi: 10.1083/jcb.201901097. PMID: 31719114
2019
Guillermier C, Doherty SP, Whitney AG, Babaev VR, Linton MF, Steinhauser ML*, Brown JD. Imaging mass spectrometry reveals heterogeneity of proliferation and metabolism in atherosclerosis. JCI Insight. 2019. Jun 6;4(11):e128528. PMID: 31167964*co-senior author
Fazeli PK, Lee H, Steinhauser ML. Aging is a powerful risk factor for type 2 diabetes mellitus independent of body mass index. Gerontology. 2019. Sep 10:epub. PMID: 31505500
Liu H, Zhang CH, Ammanamanchi N, Suresh S, Lewarchik C, Rao K, Uys GM, Han L, Abrial M, Yimlamai D, Ganapathy B, Guillermier C, Chen N, Khaladkar M, Spaethling J, Eberwine JH, Kim J, Walsh S, Choudhury S, Little K, Francis K, Sharma M, Viegas M, Bais A, Kostka D, Ding J, Bar-Joseph Z, Wu Y, Yechoor V, Moulik M, Johnson J, Weinberg J, Reyes-Múgica M, Steinhauser ML, Bernhard Kühn. Control of cytokinesis by β-adrenergic receptors indicates an approach for regulating cardiomyocyte endowment. Sci Transl Med. 2019. Oct 9;11(513). PMID: 311597755
Gyngard F, Steinhauser ML. Biological explorations with nanoscale secondary ion mass spectrometry. J Anal Atom Spectrom. 2019; 34, 1534-1545. PMID: 34054180
2018
Lu J, Baccei A, Lummertz da Rocha E, Guillermier C, McManus S, Finney LA, Zhang C, Steinhauser ML, Li H, Lerou PH. Single-cell RNA sequencing reveals metallothionein heterogeneity during hESC differentiation to definitive endoderm. Stem Cell Res. 2018. 28: 48-55. PMID: 29427839
Wildburger NC, Gyngard F, Guillermier C, Patterson BW, Elbert D, Mawuenyega KG, Schneider T, Green K, Roth R, Schmidt RE, Cairns NJ, Benzinger TLS, Steinhauser ML, Bateman RJ. Amyloid-b plaques in clinical Alzheimer’s Disease brain incorporate stable isotope tracer in vivo and exhibit nanoscale heterogeneity. Front Neurol. 2018. 9(169): 1-12. PMID: 29623063
Vujic A, Lerchenmuller C, Wu TD, Guillermier C, Rabolli CP, Gonzalez E, Senyo SE, Liu X, Guerquin-Kern JL, Steinhauser ML, Lee RT, Rosenzweig A. Exercise induces new cardiomyocyte generation in the adult mammalian heart. Nat Commun. 2018. 9(1): 1659. PMID: 29695718
Steinhauser ML, Olenchock BA, O’Keefe J, Lun M, Pierce KA, Lee H, Pantano L, Klibanski A, Shulman GI, Clish CB, Fazeli PK. The circulating metabolome of human starvation. JCI Insight. 2018. 23(16). PMID: 30135314
Zhang Y, Federation AJ, Kim S, O’Keefe J, Lun M, Xiang D, Brown JD, Steinhauser ML. Targeting nuclear receptor NR4A1-dependent adipocyte progenitor quiescence promotes metabolic adaptation to obesity. J Clin Invest. 2018. 128(11): 4898-4911. PMID: 30277475
Cox A, Tsomides A, Yimlamai D, Hwang K, Miesfeld J, Galli G, Fowl B, Fort M, Ma K, Sullivan M, Hosios A, Snay E, Yuan M, Brown K, Lien E, Chhangawala S, Steinhauser ML, Asara JM, Houvras Y, Link BA, Vander Heiden MG, Camargo F. Goessling W. Yap regulates glucose utilization and sustains nucleotide synthesis to enable organ growth. EMBO J. 2018. 37(22): e100294. PMID: 30348863
2017
Guillermier C, Poczatek JC, Taylor WR, Steinhauser ML. Quantitative imaging of deuterated metabolic tracers in biological tissues with nanoscale secondary ion mass spectrometry. Int J Mass Spectrom. 2017. 422: 42-50. PMID: 29276427
Chu AY, Deng X, Fisher VA, Drong A, Zhang Y, Feitosa MF, Liu CT, Weeks O, Choh AC, Duan Q, Dyer TD, Eicher JD, Guo X, Heard-Costa NL, Kacprowski T, Kent JW Jr, Lange LA, Liu X, Lohman K, Lu L, Mahajan A, O'Connell JR, Parihar A, Peralta JM, Smith AV, Zhang Y, Homuth G, Kissebah AH, Kullberg J, Laqua R, Launer LJ, Nauck M, Olivier M, Peyser PA, Terry JG, Wojczynski MK, Yao J, Bielak LF, Blangero J, Borecki IB, Bowden DW, Carr JJ, Czerwinski SA, Ding J, Friedrich N, Gudnason V, Harris TB, Ingelsson E, Johnson AD, Kardia SL, Langefeld CD, Lind L, Liu Y, Mitchell BD, Morris AP, Mosley TH Jr, Rotter JI, Shuldiner AR, Towne B, Völzke H, Wallaschofski H, Wilson JG, Allison M, Lindgren CM, Goessling W, Cupples LA, Steinhauser ML*, Fox CS. Multiethnic genome-wide meta-analysis of ectopic fat depots identifies loci associated with adipocyte development and differentiation. Nat Genet. 2017. 49: 125-30. PMID: 27918534 *co-corresponding author
Guillermier C, Fazeli PK, Kim S, Lun M, Zuflacht JP, Milian J, Lee H, Francois-Saint-Cyr H, Horreard F, Larson D, Rosen ED, Lee RT, Lechene CP, Steinhauser M. L., Imaging mass spectrometry demonstrates age-related decline in human adipose plasticity. JCI Insight. 2017 Mar 9;2(5):e90349. doi: 10.1172/jci.insight.90349. PMID: 28289709
2016
Kumar S, Rajagopalan S, Sarkar P, Dorward DW, Peterson ME, Liao HS, Guillermier C, Steinhauser ML, Vogel SS, Long EO. Zinc-Induced Polymerization of Killer-Cell Ig-like Receptor into Filaments Promotes Its Inhibitory Function at Cytotoxic Immunological Synapses. Mol Cell. 2016 Apr 7;62(1):21-33. doi: 10.1016/j.molcel.2016.03.009. PMID: 27058785
Hosios AM, Hecht VC, Danai LV, Johnson MO, Rathmell JC, Steinhauser ML, Manalis SR, Vander Heiden MG. Amino Acids Rather than Glucose Account for the Majority of Cell Mass in Proliferating Mammalian Cells. Dev Cell. 2016 Mar 7;36(5):540-9. doi: 10.1016/j.devcel.2016.02.012. PMID: 26954548
2015
Fazeli PK, Lun M, Kim SM, Bredella MA, Wright S, Zhang Y, Lee H, Catana C, Klibanski A, Patwari P, Steinhauser ML. FGF21 and the late adaptive response to starvation in humans. J Clin Invest. 2015 Nov 3;125(12):4601-11. doi: 10.1172/JCI83349. PMID: 26529252
2014
Steinhauser, M. L., Guillermier, C., Wang, M., & Lechene, C. P. (2014). Approaches to increasing analytical throughput of human samples with multi-isotope imaging mass spectrometry. Surface and Interface Analysis : SIA, 46(Suppl 1), 165–168. PMID: 26379341
Steinhauser, M. L., Guillermier, C., Wang, M., & Lechene, C. P. (2014). Quantifying cell division with deuterated water and multi-isotope imaging mass spectrometry (MIMS). Surface and Interface Analysis : SIA, 46(Suppl 1), 161–164. PMID: 26379340
Kim, S. M., Lun, M., Wang, M., Senyo, S. E., Guillermier, C., Patwari, P., & Steinhauser, M. L. (2014). Loss of White Adipose Hyperplastic Potential Is Associated with Enhanced Susceptibility to Insulin Resistance. Cell Metabolism, 20(6), 1049–1058. PMID: 25456741
Guillermier, C., Steinhauser, M. L., & Lechene, C. P. (2014). Quasi-simultaneous acquisition of nine secondary ions with seven detectors on NanoSIMS50L: application to biological samples. Surface and Interface Analysis : SIA, 46(Suppl 1), 150–153. PMID: 28649151